Immunochemical isolation of prolactin messenger RNA.

Isolation and characterization of collagen messenger RNA*.

Chick embryo collagen-synthesizing polysomes were isolated by differential centrifugation. RNA extracted from these particles was chromatographed in oligo(dT)-cellulose solumns and the mRNA thus obtained characterized as collagen mRNA by its electrophoetical mobility in acrylamide gels (equivalent to 1.05 x 10-6 daltons) and its effect upon a cell-free system derived from Krebs ascites tumor c...

Isolation of rat liver albumin messenger RNA.

Rat liver albumin messenger RNA has been purified to apparent homogeneity by means of polysome immunoprecipitation and poly(U)-Sepharose affinity chromatography. Specific polysomes synthesizing albumin were separated from total liver polysomes through a double antibody technique which allowed isolation of a specific immunoprecipitate. The albumin-polysome immunoprecipitate was dissolved in dete...

Concurrent isolation of ribosomal, messenger, and low molecular weight RNA.

The analysis of the cellular and molecular metabolism of anticancer drugs has provided important information for optimizing the design of clinical trials (9). In recent years, there has been an increasing use and development of RNAdirected chemotherapeutic agents, such as ribonucleotide analogs (3,5,11,13), actinomycin D (14), and flavopiridol (7). To understand their mechanisms of action, it i...

Release, identification, and isolation of messenger RNA from mammalian ribosomes.

The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA from various cells available for isolation on a large scale, and has several advantages over proc...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*